Darby: A six-year-old girl who loves to solve mysteries with her dear friends in the Hundred Acre Wood, Darby brings a fresh face to the old classic. She is a modern day kid, and she even has her own pink scooter. Darby leads the Super Sleuths as they work to solve the mysteries that perplex the inhabitants of the Hundred Acre Wood.
Buster: Buster is Darby's adorable puppy. He tags along with the team of Super Sleuths on their fact-finding adventures.
Pooh: Everyone knows Winnie the Pooh! Back again in this new, CG animated series, Pooh is as gentle, patient, and caring as ever. And now, he is one of the esteemed Super Sleuths, solving mysteries around the wood. Oh, and he still loves honey, too.
Tigger: Tigger is his name and bouncing is his game, along with solving mysteries as a Super Sleuth. Tigger is upbeat and fun, but sometimes he jumps to conclusions a little too quickly. Good thing Darby is there to help him take his time and "think, think, think."